組蛋白H3(H3)多克隆抗體
Polyclonal Antibody to Histone H3 (H3)
- 編號PAA285Bo01
- 物種Bos taurus; Bovine (Cattle，牛)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原-
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應用WB; IHC
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Bovine Cerebrum Tissue; Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Bovine Cardiac Muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Bovine Spinal cord Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Bovine Cerebellum Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Bovine Small intestine Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Bovine Colon Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Bovine Small intestine Tissue
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Bovine Cerebellum Tissue
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Bovine Cardiac Muscle Tissue
Primary Ab: 20μg/ml Rabbit Anti-Bovine H3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Spleen lysate; Primary Ab: 1μg/ml Rabbit Anti-Bovine H3 Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 1μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Cerebrum lysate;
Primary Ab: 1μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Kidney lysate; Lane3: Rat Stomach lysate; Lane4: Mouse Liver lysate; Lane5: Mouse Kidney lysate; Lane6: Mouse Stomach lysate
Primary Ab: 0.05μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Spleen lysate;
Primary Ab: 1μg/ml Rabbit Anti-Bovine H3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對H3的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別H3。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
相關產(chǎn)品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA285Bo01 | 組蛋白H3(H3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA285Bo01 | 組蛋白H3(H3)多克隆抗體 | WB; IHC |
參考文獻
| 雜志 | 參考文獻 |
| Biomed Res Int | A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome[PubMed: 25649890] |
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death[Pubmed:35732625] |