Smad同源物3(SMAD3)多克隆抗體
Polyclonal Antibody to SMAD family member 3 (SMAD3)
JV15-2; MADH3; Mothers Against Decapentaplegic Homolog 3
- 編號PAC123Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPC123Hu01-Smad同源物3(SMAD3)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 630¥ 1470¥ 2100¥ 5250¥ 21000
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
IHC圖
Figure. Western Blot; Sample: Recombinant Smad3, Human.
Western Blot; Sample: Rat Pancreas lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type PC3 cell lysate;
Lane 2: Smad3 knockout PC3 cell lysate;
Predicted MW: 26,36,43,48kd
Observed MW: 43kd
Primary Ab: 1μg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human PC3 cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對SMAD3的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別SMAD3。
用法
Western blottin:g: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC123Hu01 | Smad同源物3(SMAD3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC123Hu01 | Smad同源物3(SMAD3)多克隆抗體 | WB |
| FAC123Hu01 | 抗Smad同源物3(SMAD3)多克隆抗體 | FCM |
| MAC123Hu21 | Smad同源物3(SMAD3)單克隆抗體 | WB; IHC |
| MAC123Hu22 | Smad同源物3(SMAD3)單克隆抗體 | WB; IHC |
| MAC123Hu23 | Smad同源物3(SMAD3)單克隆抗體 | WB; IHC |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| D￡?ˉ | Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model[] |